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The effect of astragali and angelica on renal cell transdifferentiation and MAPK pathway in chronic puromycin aminonucleoside nephrosis(PAN)

WANG Rong, LI Xiao Mei, LI Jing Zi, WANG Hai Yan (Renal Division, Dept of Medicine, First Hospital and Institute of Nephrology, Peking University, Beijing 100034)  
AIM AA treated PAN rats, and to observe the effects of AA on MAPK signaling pathway. METHODS Rats were divided into control, PAN, AA treated PAN (AA) and enapril treated PAN (ACEI) groups. The pathological lesion was observed under a light microscope. Immunohistochemistry combined with semi quantitive method was used to investigate the following parameters: cell number, α SMA expression and extracellular matrix deposition. Expression and phosphorylation of protein kinases ERK, JNK and p38 were assayed. RESULTS In PAN rats, AA suppressed α SMA expression, which was closely correlated to cell proliferation, and extracellular matrix accumulation in glomerular mesangium. AA significantly attenuated α SMA expression in the tubulo interstitial area which was also parallel to the renal interstitial fibrosis.In this study, expression of all subtypes of MAPK had no difference between control and PAN groups. Compared with the inactivation of ERK and p38, phosphorylation of JNK was observed in glomeruli, renal tubules and interstitial cells in PAN rats, which was also inhibited by AA treatment. CONCLUSION The inhibitory effect of AA on phenotypic changes of renal resident cells, especially glomerular mesangial cell, may participate in its renal protective mechanisms. This effect, at least partially, was mediated by down regulated JNK activation.
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