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Analysis and Identification of Ophiopogon japonicus and Its Adulterants Using ITS2 Sequences

ZHENG Si-hao1,SUN Zhi-ying2,HUANG Lin-fang1(1.The Key 4Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193 China;2.College of Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)  
Objective:To analysis the DNA barcodes of Ophiopogonis Radix and its adulterants,and to ensure the quality and clinical safety of drugs.Methods:Download the ITS2 sequences of Ophiopogonis Radix and its adulterants from GenBank database,and the genetic distances were computed using MEGA 4.0 in accordance with the Kimura 2-Parameter(K2P) model,and the phylogenetic tree was constructed.The ITS2 secondary structure was predicted using the ITS2 database and website founded by Schultz et al.Results:Ophiopogonis Radix and its adulterants can be identified by the differences of the ITS2 sequences.The phylogenetic tree and the ITS2 secondary structure can also distinguish between Ophiopogonis Radix and its adulterants.Conclusion:ITS2 barcode sequences can serve as a valuable marker to identifying Ophiopogonis Radix and its adulterants,and it has a broad application prospects in the field of Traditional Chinese Medicine.
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