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The Inhibition of Refined Components of Medicago Sativa Polysaccharides to the Activities of Reverse Transcriptase of HIV and Protease of HIV

Zhang Liping Zhang Dongjie Feng Kun (Heilongjiang August First Agricultural Reclamation University Food college,Daqing 163319)  
Objective: To research the rate gained from the refined components, to research the inhibited result of the refined components in vitro. Methods: The coarse medicago sativa polysaccharides was soaked with hot water and flushed away in subsection with DEAE-Cellulose column. As a result, four components S1?S2?S3 and S4 were gained; Then, the activities of the reverse transcriptase of HIV and the protease of HIV were detected and compared through the test kit. Result: S3 and S4 were higher inhibition rate to the reverse transcriptase of HIV, 99.6% and 76.7% respectively; S1 and S2 were higher of the protease of HIV, 58.6% and 55.9% respectively. Conclusion: S3 and S4 showed a higher inhibiting function to the activity of the reverse transcriptase of HIV. In contrast, S1 and S2 showed a higher inhibiting function to the activity of the protease of HIV.
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