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The regulatory roles of sRNA rli106 in adaptability of Listeria monocytogenes to environmental stress and biofilm formation

GUO Yun;JI Chun-hui;WANG Li-xia;NING Cheng-cheng;LI Na;MENG Qing-ling;QIAO Jun;CAI Xue-peng;College of Animal Science and Technology, Shihezi University;China Institute of Veterinary Drugs Control Beijing;  
Listeria monocytogenes(LM) is an important food-borne pathogen that can adapt to different stressful environments. Here, to understand the biological function of LM sRNA rli106 gene, sRNA rli106 gene was amplified by PCR and its molecular characteristics were analysed. Then, LM-Δrli106 gene deletion strain and CLM-Δrli106 gene complementaion strain were constructed using homologous recombination technique. The adaptability in different stress environments, motility and biofilm formation ability of these strains were analyzed and compared, as well as the relative transcriptional levels of genes(gltB and gltC) related to biofilm generation by qRT-PCR. The results revealed that the sRNA rli106 gene was 225bp in length,containing-10 box and-35 box promoter regions in the upstream sequence, and there were the transcriptional regulator CpxR binding sites. The adaptation of LM-Δrli106 to environmental stresses at 42℃, 5% NaCl, 8% NaCl, 5 mmol/L H2 O2 was significantly reduced compared to the parental strain LM EGD-e, and there was no significant difference in motility, and the biofilm formation ability of LM-Δrli106 was significantly reduced at different periods. The relative transcription levels of gltB and gltC genes were decreased as revealed by qRT-PCR. Overall, these results for the first time confirmed that sRNA rli106 gene plays important roles in regulating the adaptability of LM to environmental stresses and biofilm formation, but does not play a role in motility. Our findings laid a foundation for revealing the molecular regulatory mechanism of rli106 in the adaptability of LM to environmental stresses and biofilm formation.
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