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Isolation and genetic evolution analysis of bovine viral diarrhea virus YBHC strain

CHEN-Shan;MA Hao-yuan;FU Jing-feng;LI Qiang;LI Xiang-zi;GAO Xu;Department of Veterinary Medicine, Agricultural College, Yanbian University;  
Yanbian Korean Autonomous Prefecture has a particular geographical location, and the trade of border livestock products is frequent. To understand the epidemic strains of bovine viral diarrhea virus(BVDV) in Yanbian, feces samples from cattle suspected of being infected with BVDV were collected from large-scale cattle farms in Yanbian Prefecture. MDBK cells were inoculated with the samples after routine treatment, and the cell cultures were used for blind passages. Although one of the fifth passage samples was positive by PCR, MDBK cells infected with the fifth passage showed no cytopathic effect. Typical BVDV virions with a size of 40nm-60nm were observed under a phosphotungstic acid negative staining electron microscope. The results of genetic evolution analysis showed that the BVDV isolate, which was a non-cytopathic type(NCP), belongs to subtype 1b and was named BVDV-1b strain YBHC. This study isolated and identified a BVDV epidemic strain in the Yanbian area for the first time. The results of this study provide the material and theoretical basis for BVDV epidemic prevention and control as well as vaccine development.
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