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Development of SCARs from AFLP Markers Linked to Resistance to Maize Rough Dwarf Virus(MRDV) Using Bulked Segregant Analysis in Maize

SHI Li-yu1,2,LI Xin-hai2,XIE Chuan-xiao2,HAO Zhuan-fang2,WENG Jian-feng2,ZHANG Shi-huang2,PAN Guang-tang1(1Maize Research Institute,Sichuan Agricultural University,Ya'an 625014,Sichuan;2Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility of Crop Gene Resources and Genetic Improvement,Beijing 100081)  
【Objective】Developing and cultivating resistant hybrids is an effective approach to control MRDD.Marker development and markers-assisted selection(MAS) is an efficient way for MRDD-resistant breeding.【Method】On the basis of MRDD resistance evaluation of 152 maize inbred lines,resistant and susceptible DNA bulks were composed by using genomic DNAs of 10 resistant and 10 susceptible inbred lines,respectively.Polymorphic AFLP markers were screened between two bulks and then transformed into SCAR(sequence characterized amplified region) markers.Identification of correlation between the SCAR markers and MRDD resistance was carried out in 152 inbred lines.【Result】SCAR69 and SCAR74 were validated to be highly associated with MRDD resistance.【Conclusion】SCAR69 and SCAR74 could be used for MAS of MRDD resistance in maize.
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