Bioinformatics and Expression Analysis of HaLACS7 Gene in Sunflower
Zhou Fei;Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme;Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences;
LACS encodes for long-chain acyl-CoA synthetase, a protein that converts free fatty acids into long-chain acyl-CoA, an essential lipid metabolite. The bioinformatics analysis of the HaLACS7 encoded protein in sunflower was carried out in this study. The protein encoded by HaLACS7 was expected to be an acidic and hydrophilic protein. The protein encoded by HaLACS7 had an irregular coil secondary structure, and it's subcellular location was anticipated to be in the peroxisome. With 84% sequence similarity, HaLACS7 was shown to be closely related to Lactuca sativa L. and Cynara cardunculus var. Scolymus L. LACS6, and clustered in a broad branch with LACS6 and LACS7 of Arabidopsis thaliana L. The expression level of HaLACS7 in sunflower tissues was ligulate flowers root seed tubular flower leaf stem. The expression level of HaLACS7 was higher at the late development stage of high and low oleic acid sunflower seeds, but the expression trend was significantly different at different seed development stages of the two materials. Therefore,it was suggested that the expression level of HaLACS7 may affect the synthesis of oleic acid in sunflower seeds.