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Genome Size Estimation for Two Campanulaceae Plants

FAN Huijie;GONG Qiang;HUANG Haoying;CHEN Lele;CHAI Zhi;ZHOU Ran;Shanxi University of Chinese Medicine;  
Objective To investigate the genome sizes of traditional medicinal herbs Dangshen[Codonopsis pilosula(Franch.) Nannf.]and Jiegeng[Platycodon grandiflorum(Jacq.) A. DC.]from Campanulaceae. Methods The fresh leaves of Dangshen[Codonopsis pilosula(Franch.) Nannf.]and Jiegeng[Platycodon grandiflorum(Jacq.) A. DC.]were picked as the materials of experiment, and Menggu Huangqi[Astragalus membranaceus(Fisch.) Bge. var. mongolicus(Bge.) Hsiao]was chosen as an internal reference of Dangshen[Codonopsis pilosula(Franch.) Nannf.]and Dadou[Glycine max(Linn.) Merr.]was chosen as an internal reference of Jiegeng[Platycodon grandiflorum(Jacq.) A. DC.]. The nuclear suspension was prepared by lysis buffer and was stained by propidium iodide. Flow cytometry was used to detect the fluorescence intensities of Dangshen[Codonopsis pilosula(Franch.) Nannf.]and Jiegeng[Platycodon grandiflorum(Jacq.) A. DC.]. Results The genome size of Dangshen[Codonopsis pilosula(Franch.) Nannf.]was 1.00 Gb, and that of Jiegeng[Platycodon grandiflorum(Jacq.) A. DC.]was 0.70 Gb. Conclusion The genome sizes of Dangshen[Codonopsis pilosula(Franch.) Nannf.]is larger than that of Jiegeng[Platycodon grandiflorum(Jacq.) A. DC.]and the difference is about 0.30 GB, which provides important support for the differences between Dangshen[Codonopsis pilosula(Franch.) Nannf.]and Jiegeng[Platycodon grandiflorum(Jacq.) A. DC.]in the same family.
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