The effect of anthraquinone glycoside from Polygonum multiflorum Thunb on cellular immunological function in mice
Sun Guibo,Guo Baojiang,Li Xueer ,Huang Junni, Xue Huebao,Sun Xiaobao (Guangdong Key Lab of Biotechnology for Plant Development,South China Normal University, Guangzhou 510631; 1Academy of Traditional Chinese Medicines and Materia Medica of Jilin Province.Changchun 13002l)
Objective:To study the effect of anthraquinone glycoside from Polygonum multiflorum Thunb.(AGPMT) in vitro on cellular immunological function in mice. Methods:The mouse lymphocytes or macrophages were co-cultured with AGPMT.T and B lymphocyte proliferation,mixed lymphocyte reaction(MLR) and the antagonistic effect on reduced lymphocyte proliferation by mitomycin(Mit C) was determined by XTT method. Macrophages phagocytosis,tumor necrosis factor (TNF) secretion activity of mouse spleen cells and activity of natural killer(NK) cells was determined by using neutral red assay. Results: AGPMT could significantly accelerate T and B lymphocytes proliferation in vitro,improve macrophages phagocytosis , increase TNF secretion activity and activity of NK cells, accelerate mixed lymphocyte reaction, antagonize restraining effect of lymphocyte proliferation by Mit C. Conclusion: AGPMT could potentiate the immunological function.