In Vitro Study of Chemotherapy-sensitizing Effect of Astragaloside Ⅳ on Breast Cancer Stem Cell during
XIE Hai-mei;LI Hua-chao;CHEN Qian-jun;Guangdong Provincial Hospital of Traditional Chinese Medicine;
Objective To investigate the effect of astragaloside-Ⅳ（ASIV）on chemotherapy sensitivity of breast cancer stem cells（BCSCs）. Methods Breast cancer SUM159 cells were cultured in vitro and SUM159 stem cells were induced by suspension culture. During the experiment,the stem of SUM159-CSCs was identified firstly by the proportion of CD44+/CD24-cell group detected by flow cytometry,and by the re-differentiation and balloon formation. Secondly,the drug resistance of SUM159-CSCs to paclitaxel（PTX）was observed:the cell viability was detected by CCK8 test, the apoptosis was detected with flow cytometry, cell ball-forming activity was detected by 3D balloon test. Finally,SUM159-CSCs was divided into control group,ASIV group,PTX group and ASIV + PTX group. And then cell viability was detected by CCK8 test, apoptosis was detected by flow cytometry,cell ball-forming activity was detected by 3D balloon test,and the expression of apoptotic protein Bax and Bcl-2 was detected by Western blotting method. Results The resistance of SUM159-CSCs to PTX was higher than that of SUM159（P0.05）. ASIV combined with PTX significantly inhibited the viability,proliferation and balloon viability of SUM159-CSCs cells,the expression of Bax was significantly up-regulated and the expression of Bcl-2 was significantly down-regulated. Conclusion ASIV can enhance the sensitivity of paclitaxel therapy by inhibiting the proliferation of tumor stem cells and promoting apoptosis.