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The Role of Glucosamin Sulphate in Replicative Senescence of Rat Chondrocyte

CHEN Xiaodong,LIN Jianhua (Department of Spine Surgery,Shenzhen Pingle Hospital of Orthopedics and Traumatology,Shenzhen 518010,China)  
Objective: To observe the effect of glucosamin sulphate(GS) on replicative senescence of rat chondrocyte subcultivated in vitro by means of GS interfering and controlled experiment.Methods: The 3rd passage chondrocytes were divided into the blank group,different concentration GS groups passaged to 4th generation,the control group of the 2nd passage.The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,for cell life cycle and proliferation index with flow cytometry,for the content and constructure of GAG of ECM with alcian blue test,and for type Ⅱcollagen and Aggrecan with RT-PCR.A molecular biology study was also conducted on GS' preventive role against chondrocyte senescence.Results: GS can significantly inhibit chontrocyte's expression of S-A-β-gal,promote chontrocyte's proliferating(P0.01),reduce cell content in G1 phase,increase content of GAG,and enchance the expression of type Ⅱcollagen and Aggrecan of ECM(P0.01).Conclusion: GS can significantly oppose rat chondrocyte senescence occurred in subcultivation.
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