Effects of diosgenin on cell proliferation,differentiation and OPG/RANKL mRNA expression of rat osteoblasts cultured in vitro
JIANG Yang-zhen, WANG Jun-jun, LIANG Ji-chao, CHEN Yong (Hubei Province Key Laboratory of Biotechnology of Chinese Materia Medica, Hubei University, Wuhan 430062,China)
Objective:To study the effects of diosgenin on cell proliferation, differentiation and OPG/RANKL mRNA expression of rat osteoblasts cultured in vitro for analyzing the prevention and treatment mechanism of diosgenin. Methods: Rat osteoblast cultured in vitro was treated by a series of different concentrations of diosgenin for different time respectively. The proliferation of osteoblast was detected by MTT method. The differentiation of osteoblast was detected by the activity of ALP mRNA expression of osteoprotegerin(OPG) and RANKL(receptor activator nuclear factor-kappa B ligand,RANK) were semi-quantified by RT-PCR, and the ratio of OPG/RANKL mRNA was used to evaluated the effect of diosgenin on osteoclast signal transduction pathway of OPG/RANKL/RANK system. Results: Diosgen at the concentration of 3.64×10-8 and 3.64×10-7mol/L can promote the proliferation, enhance the activity of ALP and raise the ratio of OPG/RANKL mRNA of rat osteoblast cultured in vitro(P0.05 or P0.01). Conclusion: The suitable concentration of diosgenin could promote the proliferation, differentiation of rat osteoblast and inhibit the formation of rat osteoclast.