Study on the Technology of Tissue Culture in Aloe Vera Var Chinensis
Liu Yongti, Zhong Zhengbin (Yichang Prefecture Institute of Forestry Yichang 443111)
Take young stems of Aloe vera var chinensis as explant ,breed additional various kinds and hormone MS of concentration or based on improved MS with culture medium while adding 6- BA 1. 5mg/L, the effect of inducement of overgrown bud is optimum and sprout quantity is the most; growth of overgrown bud is the best and bud is length and also healthy while adding 6- BA 1. 0mg/L + NAA 0.3 mg/L; when seedlings height are above 3 cm and the root length reaches over 3 cm, the effect of root develops well while adding NAA 0.2 mg/L; open lid for 3 days and then move it into greenhouse (10- 16 °C), and train seedlings after 3 days and then transplant them into vermiculite; sandy bed; humus soil with mixture medium soil by 0. 5:1:1.5, and survival rate reaches 91.3%.