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Study on Cell Agglutination and Sugar-inhiblting Action of the Lectin from Brassica oleracea var.botrytis L.

Huang Deqi Yu Ping Zhu Sumin Yan Zhisen Lin Yuman (Institute of polymer Science)  
The lectin from Brassica oleracea var. botrytis L. agglutinated theerythrocytes from such species as rabbit, rat and mouse, and its agglutinating activity on the erythrocytes from rabbit was the highest among them because it had the lowest agglutinating concentration of 0.68μg/ml. But the lectin did not agglutinate the erythrocytes from other 16 species tested. In addition, the lectin at a concentration of 0.1mg/ml also exhibited hemagglutination activity on some tumor cells such as Ehrlieh ascite cells and S_(180) tumor cells from mouse, W_(256) tumor cells from rat and MGC_(80-3) tumor ceils from man, the peripheral lymphocytes and bone marrow cells from mouse and rat, and the sperm cells from cow. But it did not agglutinate Hela tumor cells from man. The hemagglutination of the lectin from Brassica Oleracea ear. botrytis L. on rabbit erythrocytes was inhibited by L—rhamnose and D—arabinose among the sugar tested and they respectively had the lowest inhibiting of concentration 33.3 m mol/L and 16.7 m mol/L.
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