The effect of 595nm Vbeam laser on proliferative activity and apoptosis of fibroblasts in the hypertrophic scarring models in rabbit ears
TENG Wen,HAO Li-jun,REN Li-hong,XIAO Zhi-bo,WANG Min,LI Shao-hao (Department of Laser & Cosmetic, 2nd Affiliated Hospital of Harbin Medical University, Harbin 150086 ,Heilongjiang China)
Objective To investigate the effect of 595nm Vbeam laser on proliferative activity and apoptosis of fibroblasts in the hypertrophic scarring models in rabbit ears. Methods 20 white rabbits were used to establish the models of hypertrophic scar in ears. To study the changes of fibroblasts with 595nm Vbeam laser irradiation and compare with the controls during the process of forming hypertrophic scars. The expression of proliferation cell nuclear antigen (PCNA) protein was measured by immunohistochemistry technique, and the apoptosis was measured by TUNEL technique. Results After 595nm Vbeam laser irradiation, the material was taken on different time. The expression of PCNA was obviously weaker than the controls, and the apoptosis was increased in the laser irradiation group. Conclusion 595nm Vbeam laser irradiation can inhibit the proliferation of fibroblasts and accelerate apoptosis in hypertrophic scars of rabbit ears and has significant effect on hypertrophic scars.
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