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Effects of eucalyptus oil on the growth of human chorionic cells cultured in vitro

XIANG Tao1,LI Xiao-yan,YU Yong-li,CHENG Qin,XU Ben-hong,ZHANG Ya-ling,LI Xiao-hong(1 Jin Wan Area Family Planning Service Station,Zhuhai 519041,P.R.China)  
Objective To investigate the effect of eucalyptus oil on the growth of human chorionic cells cultured in vitro,and to provide the basis for its application in food additives and drug development.Methods The normal pregnancy(in 6-8 weeks)human chorionic cells were collected with the trypsin digestion method,then randomly divided into eucalyptus oil group and control group,the eucalyptus oil group was given eucalyptus oil to make final mass concentration reach 0.75,0.15,0.015,0.001 5 mg/mL;The control group was treated with 70% ethanol 10 μL/mL.The cell growth curve was drawn with the trypan blue staining and cell notation;MTT method was used for determination of cell proliferation inhibition rate 12,24 and 36 hs after the eucalyptus oil was processed,flow cytometry instrument was used to detect the cell cycle and apoptosis situation 12 and 24 hs after.Results ① With the extension of incubation time,the number of human chorionic cells in the two groups both reduced gradually,the human chorionic cell growth in the 0.75 mg/mL eucalyptus oil group was obviously inhibited and showed significant differences compared with that in control group.② The proliferation inhibition rate of human chorionic cells in the 0.75 mg/mL eucalyptus oil group was significantly higher than that in the control group(P0.05),the proliferation inhibition rate of the other eucalyptus oil groups showed no significant difference comparing with that in the control group.③ Twelve hours after the treatment of eucalyptus oil,G0-G1 phase cells in the 0.75 mg/mL eucalyptus oil group reduced,S period cells increased,G2-M phase cells reduced,apoptosis rate significantly increased,comparing with the control group,P0.05;Compared with the control group,G0-G1 phase and G2-M phase cell proportion in the rest eucalyptus oil groups showed significant differences(P0.05),S period cell apoptosis rate and proportion showed no significant difference.Twenty-four hours after the treatment of eucalyptus oil,G0-G1 phase and S period cells in 0.75 mg/mL eucalyptus oil group reduced,G2-M phase cells and apoptosis rate increased(P0.05),compared with the control group;The S period cell proportion in the rest eucalyptus oil groups increased significantly(P0.05),G2-M cell proportion and apoptosis rate showed no significant difference.ConclusionsEucalyptus oil of 0.15 mg/mL or lower has no obvious roles of growth inhibition,cell toxicity and apoptosis induction on the human chorionic cells,but it can affect the cell cycle,and this effect may different vary with different action time;This provides the basis for the application of eucalyptus oil in food additives and drug development.
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