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Effect of Relaxing Large Intestine Therapy on Contents of SP and VIP in Lung Tissues of Rat Models with Chronic Obstructive Pulmonary Diseases

Zhang Tianyu;Zhang Jinchao;Liu Miao;Wu Ruohan;Zhang Shujing;Gao Yushan;Zheng Fengjie;Xu Hong;Li Yuhang;School of Basic Medical Sciences,Beijing University of Chinese Medicine;  
Objective: To observe the influence of relaxing large intestine therapy on contents of substance P( SP) and vasoactive intestinal peptide( VIP) in rat model with chronic obstructive pulmonary disease( COPD) and explore the effective mechanism of treating COPD based on relaxing the large intestine from the aspect of neuropeptide. Methods: Rats model of COPD were established through intratracheal injection of LPS combined with cigarette smoking. Fifty Wistar rats were randomly divided in to normal group,model group, intestine group,lung group and lung-intestine group. The normal group and model group were intragastrically given normal saline solution and other groups were treated with corresponding decoction intragastrically for 7 days. The content of SP and VIP were detected by immunohistochemistry and enzyme-linked immunosorbent assay. Results: Immunohistochemistry results showed that compared with control group,SP in lung tissue of model group was abundantly expressed while in lung group and intestine group and lung-intestine group were weakly expressed. VIP in bronchial epithelium of control group was expressed broadly while model group were lower. After treatment,VIP was increased significantly in trial groups. Enzyme-linked immunosorbent assay showed that compared with control group,SP was increased in lung tissue in model group while VIP were decreased significantly( P 0. 01). Compared with model group,SP was lower in lung group,intestine group and lung-intestine group. VIP was increased significantly( P 0. 01 or P 0. 05). Compared with lung group,SP was reduced significantly in lung-intestine group( P 0. 05). Conclusion: The expression of SP and VIP were regulated by the therapy of relaxing the large intestine or relaxing the large intestine on the base of treating lung disease. This may be one of the mechanisms of why treating from intestine is effective on COPD.
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