STUDIES ON MACHANISM ABOUT ATP INHIBITED THE PROLIFERATION OF MGC-803 CELLS
Lu Gui-zhi Lin Zhong-xiang Zhang Zhi-qian Zhou Li-xin Xu Guang-wei (Beijing Institute for Cancer Research, Beijing, China 100034)
This experiment was to study the ma-chanism of ATP anticancer effects. By using flow cytometry, Scrape-Loading and dye transfer (SLDT), dot hybridization methods, changes of cell cycle phase distribution, gap junctional intercellular communication (GJIC), and oncogene expression were observed in human stomach mucous glandular carcinoma (MGC-803) cells treated with ATP (0.23mg/ml). It was found that ATP inhibited the proliferation and arrested cell cycle in S phase. The ATP-treated MGC-803 cells increased in GJIC, and decreased in expression of c-Ha-ras oncogene. These results indicated that the inhibition of proliferation and increased GJIC was closely correlated with the reduction of c-Ha-ras oncogene expression.