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Ethyl acetate:an alternative to ether in anesthetizing Drosophila melanogaster

XU Jing-yin,WANG Zi-qi,XU Ya-li,ZHANG Yi-ding,REN Bi-jie,YANG Da-xiang(College of Biological Sciences,China Agricultural University,Beijing 100094,China)  
In biology class,diethyl ether is the most commonly used anaesthetic in Drosophila melanogaster anesthetizing.However,the availability and use of the ether is strictly controlled by law in China.Here we reported that ethyl acetate,an effective asphyxiant used in insect collecting and study,can be used as a substitute for ether in Drosophila melanogaster anesthetizing.For each trial,20-30 wild-type flies were randomly selected from culture vials,and were anesthetized in a container(125 cm3) with 40,80,120 μl ethyl acetate or diethyl ether(CK),each trial was replicated four times.The percentage of unaroused flies 20min and 120min after fully anesthetized were counted for estimating the effect and safety of ethyl acetate.The results indicated that ethyl acetate can be used to anesthetize Drosophila melanogaster;the time for ethyl acetate effect is longer than that of ether;however,the time at which the flies were fully anesthetized by ethyl acetate was shorter than or similar to that by corresponding ether control;the anesthesia time of ethyl acetate is longer than that of ether control;more than 90% ethyl acetate anesthetized flies aroused within 120min.It indicated that ethyl acetate was safe to Drosophila melanogaster in the tried concentration.Result suggested that ethyl acetate can be used as a substitute for diethyl ether in biology laboratory.
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