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Fungicide and insecticide activity of Justicia procumbens extracts

GUO Ming-Cheng1,LI Bao-Tong1,TANG Li-Mei1,PING Xin-Liang2,LI Xiao-Hua3,LIANG Tong-Jun3(College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China;2.Institute of Citrus Research,Zhejiang Academy of Agricultural Sciences,Taizhou 318020,China;3.Jiangxi Lushan Arboretum,Lushan 332900,China)  
Justicia procumbens is traditional Chinese medicine herb.To confirm fungicidal and insecticidal activity of J.procumbens extracts,methanol,chloroform,acetone,ethyl acetate and hexane extracts were obtained from J.procumbens by the combined method of cold-soaking and ultrasonic extraction.Extract inhibitory effects were studied using the inhibition zone method involving five species of plant pathogenic fungus—Colletotrichum gloeosprioides,Phomopsis asparagi,Pyricularia grisea,Fusarium graminearum and Botrytis cinerea.Methanol extract toxicity against four species of plant pathogenic fungus and bacteria(C.gloeosprioides,P.asparagi,B.cinerea and Xanthomonas campestris) and three species of insects(Aedes albopictus,Musca domestica and Pieris rapae) were also assayed.Results showed that 8 mg.mL 1extracts of J.procumbens with different polar solvents had some inhibitory effects on five species of plant pathogenic fungus.The inhibition rates of methanol extract against C.gloeosprioides,P.asparagi and B.cinerea were over 50%.Methanol extract from J.procumbens had better fungicidal and insecticidal activity.EC50 of methanol extract against mycelium growth of C.gloeosprioides,P.asparagi and B.cinerea were 5.94 mg.mL 1,4.61 mg.mL 1 and 5.27 mg.mL 1,respectively.The corresponding EC90 were 63.69 mg.mL 1,58.01 mg.mL 1 and 54.57 mg.mL 1,respectively.Methanol extract at 0.25~1.00 mg.mL 1showed strong inhibitory activity against X.campestris.The resultant extract at 0.125 mg.mL 1showed moderate inhibitory activity.Methanol extract MIC was 0.062 5 mg.mL 1.Methanol extract LC50 against A.albopictus,M.domestica and P.rapae were 0.195 8 mg.mL 1,0.351 4 mg.mL 1 and 0.287 7 mg.mL 1,respectively.The corresponding EC95were 0.988 4 mg.mL 1,3.053 2 mg.mL 1and 2.584 4 mg.mL 1,respectively.This suggested that J.procumbens extracts had better application prospects as a biological pesticide in agricultural production.
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