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TIAN Ye, LI Chao-pin(Department of Etiology , School of Medicine , Anhui University of Science and Technology, Huainan 232001, China)  
Objective To investigate the occurrence of Demodex infestation in patients with blepharitis, so as to provide the reference for diagnosis and treatment of blepharitis associated with Demodex. Methods 507 patients with blepharitis, 498 patients with other ophthalmopathy and 295 volunteers with quiet eyes as normal control group were involved into this study. Discharge and three eyelashes from each blepharon were taken from all individuals, and were examined for Demodex under microscope. Results The positive rate of Demodex was 50. 69% in patients with blepharitis, which was significantly higher than that in patients with other ophthalmopathy(11. 65 %) (P0. 01) and in normal control group (10. 51%) (P0. 01). There was no significant difference between male (51. 55%) and female (49. 54%)(P0. 05). The positive rate of Demodex was the highest in patients over 60 years (69.23%), and it was significantly different in patients of different ages (P0. 01). Conclusion The positive rate of Demodex was high in patients with blepharitis. In order to avoid prolong of the disorders of the blepharitis associated with Demodex, doctors should make their diagnosis using Demodex detection by microscopy and observation of eyelashes by naked eyes, and carry out anti-Demodex treatment when necessary.
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