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Hepatotoxicity on Water Extracts and the Total Alkaloid of Farfarae Flos

HUI Lian-qiang,GAO Shuang-rong,LIU Ting,LI Chun-ying,HAO Ran,YI Yan, GUO Jing,HE Rong,CAO Chun-yu,ZHAO Yong,LIANG Ai-hua,ZHANG Yi(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)  
Objective:To study the hepatotoxicity of Farfarae Flos.Method:KM mice were given the water extracts of Farfarae Flos orally in 20,40 g·kg-1 doses for successive 4 weeks,enzyme activity of aspartate transaminase(AST),alanine transaminase(ALT) in serum of mice were measured,coefficient of liver were calculated,and liver histopathology examination were conducted.Using the precision-cut slice technology,culture liver slices for 24 hours with water extracts in concentration 0.005,0.05 g·L-1 and 6 hours with the total alkaloid of Farfarae Flos in concentration 0.5,2.0 g·L-1,the slice homogenate were prepared,protein concentration were detected by BCA protein assay method,enzyme activity of ALT,AST,lactate dehydrogenase(LDH),r-glutamgl thanspeplidase(GGT) were detected by enzyme kinetics method and leakage were calculated.Result:The water extract of Farfarae Flos 40 g·kg-1 dose group of female had significant histopathology changes and organ coefficient of liver increased compared with control group.After co-culture for 24 hours with water extracts of Farfarae Flos,ALT leakage were significantly increased in two groups;LDH and ALT leakage were significantly increased with final concentration 0.5 g·L-1,GGT leakage was significantly increased and protein content was obviously decreased with final concentration 2.0 g·L-1 after co-culture for 6 hours with the total alkaloid of Farfarae Flos.Conclusion:Farfarae Flos displayed liver toxicity in some degree for in vivo and in vitro experiments.
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