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Normobaric Hyperoxia Induced Brain Cell Apoptosis and Delayed Early Neurobehavioral Development in Newborn Rats

LI Yi-juan1,LIU Mei-na1,YU Mu-xue1,ZHUANG Si-qi1,LIU Yong-dong2,CHE Li-hong2(1.Department of Pediatrics,2.Department of Pathology,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)  
【Objective】 To evaluate the effects of hyperoxia on the brain cell apoptosis,expression of MBP(Myelin base protein) and the neurobehavioral performance.【Methods】 A total of 166 7-day-old SD rats were randomly assigned into 2 groups: 80% hyperoxia group and normoxia group.Rat pups were killed in each group on the 24 h,72 h after the exposure of oxygen for tunnel staining and apoptotic index(AI) was calculated.After exposing to oxygen for 72 h,tests for neurobehavioral toxicity were performed from d10 to d15 and the pups were killed on d10,d15 for MBP stained with immunohistochemical method.Normoxia group was used as normal control.【Results】 The AI in 2h group was significantly higher than 0h group(normoxia group)(P 0.01).The AI of hyperoxia group was increased gradually with the prolongation of exposure to 80% oxygen and reached the highest level after exposure to oxygen for 12 ~ 24 h.After exposure to oxygen for 72 h,the AI decreased while still higher than 0 h group(P 0.01).The expressions of MBP in the brain of hyperoxia group were significantly lower than normoxia groups at d10 and d15(137 ± 5 vs.131 ± 6,128 ± 5 vs.122 ± 5)(P 0.05).The positive rates of acoustic startle,forelimb hanging,negative geotaxis,air righting were significantly lower than normoxia group at a certain age(P 0.05).【Conclusions】 Normobaric hyperoxia could induce cell apoptosis in the newborn rat.Hyperoxia could decrease the expression of MBP to interfere the myelination of the developing brain.Hyperoxia could delay the neurobehavioral development of newborn rats.
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