Study on the anti-inflammatory and analgesic activities of ethyl acetate extract from humulus scandens (Lour.)Merr
Cao Weiguo,Zhang Dan,Zhang Yibin,Liu Sha (TCM College of Chongqing University of Medical Sciences,Chongqing 401331)
Objective:To study on the anti-inflammatory and analgesic activities of ethyl acetate extract from Humulus scandens (Lour.)Merr.and its underlying mechanism.Methods:Acetic acid writhing method and hot-plate method were used to study the analgesic activities.Auricle edema induced by dimethylbenzene,cotton pellet granuloma of the mice and edema in feet induced by egg whites were applied for anti-inflammatory experiments.The underlying mechanism was performed by formaldehyde method and determination on the content of PGE2 in inflammatory tissue.Result:The ethyl acetate extract from Humulus scandens (Lour.)Merr.inhibited acid-induced mouse writhing response and enhanced the threshold of pain value significantly.The ethyl acetate extract inhibited auricle edema induced by dimethylbenzene and edema in feet induced by egg whites significantly.The ethyl acetate extract significantly inhibited the size of cotton pellet induced granuloma formation.The ethyl acetate extract had inhibitory effect onⅡphase pain in formalin inducing pain.The extract could reduce the content PGE2 in inflammatory tissue.Conclusion:The ethyl acetate extract from Humulus scandens (Lour.)Merr.shows anti-inflammatory and analgesic activities.The extract showed analgesic activity through inhibition subsequent analgesic reaction.The anti-inflammatory activities may relate to its inhibitory effects on the content of PGE2 in inflammatory tissue.